過氧化氫酶(CAT)多克隆抗體
Polyclonal Antibody to Catalase (CAT)
- 編號UPAC418Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPC418Hu01-過氧化氫酶(CAT)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Felis catus; Feline (Cat，貓)
- 應(yīng)用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 252 ¥ 588 ¥ 840 ¥ 2100 ¥ 8400
- 欲了解實(shí)際交易價(jià)格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CAT Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
-
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human CAT Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
-
Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Kidney lysate; Lane3: Mouse Liver lysate; Lane4: HepG2 cell lysate; Lane5: Hela cell lysate Primary Ab: 0.04μg/ml Rabbit Anti-Human CAT Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
-
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: CAT knockout HepG2 cell lysate;
Predicted MW: 60kd
Observed MW: 60kd
Primary Ab: 2μg/ml Rabbit Anti-Human CAT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human THP1 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human CAT Polyclonal Antibody (Catalog PAC418Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對CAT的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別CAT。
用法
Western blotting:0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC418Hu03 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC418Hu02 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC418Hu05 | 過氧化氫酶(CAT)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC418Hu05 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC418Hu04 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC418Hu02 | 過氧化氫酶(CAT)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC418Hu01 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC418Hu03 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; FCM |
| UPAC418Hu01 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| UPAC418Hu05 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; FCM |
| UPAC418Hu04 | 過氧化氫酶(CAT)多克隆抗體 | WB; ICC/IF; FCM |
| UPAC418Hu02 | 過氧化氫酶(CAT)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAC418Hu71 | 過氧化氫酶(CAT)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAC418Hu81 | 過氧化氫酶(CAT)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAC418Hu21 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC |
| UMAC418Hu27 | 過氧化氫酶(CAT)單克隆抗體 | WB |
| UMAC418Hu29 | 過氧化氫酶(CAT)單克隆抗體 | WB |
| UMAC418Hu22 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAC418Hu24 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAC418Hu23 | 過氧化氫酶(CAT)單克隆抗體 | WB |
| UMAC418Hu25 | 過氧化氫酶(CAT)單克隆抗體 | ICC/IF |
| UMAC418Hu26 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC |
| UMAC418Hu28 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC |
| USEC418Hu | 過氧化氫酶(CAT)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC418Hu | 過氧化氫酶(CAT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSC418Hu01 | 過氧化氫酶(CAT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Arteriosclerosis, Thrombosis, and Vascular Biology | Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic Aneurysm [Ahajournals: source] |
| Fundam Clin Pharmacol. | Phloroglucinol protects gastric mucosa against ethanol-induced injury through regulating myeloperoxidase and catalase activities [PubMed: 20880383] |
| Oral Disease | Antioxidant profile of salivary glands in high fat diet-induced insulin resistance rats [Pubmed: 24106991] |
| Yonsei Med J. | Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. [Pubmed: 24142649] |
| Journal of Food Science and Technology | Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar rats [Springer: Source] |
| 第 三 軍 醫(yī) 大 學(xué) 學(xué) 報(bào) | 過氧化氫酶基因 ３８９Ｃ＞Ｔ多態(tài)位點(diǎn)在重慶漢族人群中的分布及其與噪音性耳聾的 關(guān)聯(lián)研究 [Tmmu:Source] |
| Adv Med Sci | Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, [PubMed: 26674569] |
| Arch Oral Biol | Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes [PubMed: 26143097] |
| J Oral Pathol Med | Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva [PubMed: 26608886] |
| О Р И Г И Н А Л Ь Н Ы Е С Т А Т Ь И | КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИ [docs:225188] |
| Current Alzheimer Research | Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients. [pubmed:27748187] |
| Journal of Cancer Research and Treatment | Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines [jcrt:5] |
| Adv Med Sci. | Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects. [pubmed:28242484] |
| PeerJ | Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2 [pubmed:28785522] |
| Pharmacological Reports | Evaluation of ADMA, carbonyl groups, CAT and NKA in depressed patients with and without posttraumatic stress disorder. [pubmed:28554099] |
| Ecotoxicology and Environmental?Safety | Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oil [Pubmed:29660615] |
| Human and Ecological Risk Assessment: An International Journal | Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails [10.1080:10807039.2018.1460800] |
| Experimental and Therapeutic?Medicine | Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO?1 signaling pathway [Pubmed:30116359] |
| Journal of?Cosmetic?Dermatology | A red pumpkin seed extract reduces melanosome transfer to keratinocytes by activation of Nrf2 signaling [Pubmed:30047218] |
| Anticancer?Research | Cholecystectomy Patients with High Plasma Level of Catalase Have Significantly Lower Analgesia Requirement: A Prospective Study of Two Different?… [Pubmed: 30194197] |
| Anticancer?Research | Plasma Catalase in Relation to Pain Following Midline Laparotomy: A Prospective Study of Patients with Benign Diseases and Patients with Cancer [Pubmed: 30396975] |
| Journal of Integrative Medicine | Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats [Doi: 10.1016/j.joim.2018.12.002] |
| EXPERIMENTAL ANIMALS | Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formation [Pubmed: 30606939] |
| MOLECULAR BIOLOGY REPORTS | An in vitro protocol to study the effect of hyperglycemia on intracellular redox signaling in human retinal pigment epithelial (ARPE-19) cells [Pubmed: 30628023] |
| Anticancer?Research | Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients [Pubmed: 30711961] |
| Anticancer?Research | Rectus Sheath Block (RSB) Analgesia Could Enhance Significantly the Patient Satisfaction Following Midline Laparotomy in Benign Disease and in Cancer: A?… [Pubmed: 30842172] |
| Molecular Medicine Reports | MicroRNA?21 contributes to the puerarin?induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury [Pubmed: 31115556] |
| Advances in Medical Sciences | Selected elements of extracellular matrix of the skin in diabetes and insulin resistance [Pubmed: 31146169] |
| European Journal of Biomedical and Pharmaceutical Sciences | European Journal of Biomedical AND Pharmaceutical sciences [] |
| Anticancer Research | The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer [Pubmed: 31892574] |
| PHARMACOLOGICAL RESEARCH | A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 Signaling [Pubmed: 32135248] |
| Biomolecules | Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development [Pubmed: 32751983] |
| AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS [] | |
| The Journal of Physiology | In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells [] |
| Journal of Cell Communication and Signaling | Lutein reverses hyperglycemia-mediated blockage of Nrf2 translocation by modulating the activation of intracellular protein kinases in retinal pigment epithelial (ARPE?… [] |
| Plasma catalase (CAT) levels in gallstone disease: Cholecystectomy patients with high plasma levels of CAT have significantly lower number of analgesic … [] | |
| Malaysian Journal of Medicine and Health Sciences | Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring [] |
| nature environment pollution technology | Toxic Trace Metals and Pathological Changes in Organs of Rats Fed with Extract of Polluted Grasses [] |
| World Neurosurg | Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ?- [33689849] |
| Biomed Res Int | Mechanisms of Trx2/ASK1-Mediated Mitochondrial Injury in Pemphigus Vulgaris [33763469] |
| Nutrients | Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and??- [34064937] |
| Molecular Medicine Reports | Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats [34278476] |
| J Pers Med | Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis [34945751] |
| In Vivo | Plasma Concentration of the Lipid Peroxidation (LP) Biomarker 4-Ηydroxynonenal (4-HNE) in Benign and Cancer Patients [Pubmed:35241533] |
| Biochimica et Biophysica Acta-Molecular Basis of Disease | α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet [Pubmed:35569738] |
| Antioxidants | Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory?… [Pubmed:35204215] |
留言咨詢
