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脫氧核糖核酸酶Ⅰ(DNASE1)多克隆抗體

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human DNASE1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human DNASE1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human DNASE1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human DNASE1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant DNASE1, Human.
Knockout Varification: Lane 1: Wild-type 293T cell lysate; Lane 2: DNASE1 knockout 293T cell lysate; Predicted MW: 20,31kDa Observed MW: 20kDa Primary Ab: 2μg/ml Rabbit Anti-Human DNASE1 Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human DNASE1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Stomach lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human DNASE1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Colon lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human DNASE1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

該抗體是針對(duì)DNASE1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別DNASE1。

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

雜志	參考文獻(xiàn)
J Card Fail	Low Circulating Levels of Mitochondrial and High Levels of Nuclear DNA Predict Mortality in Chronic Heart Failure [Pubmed:27349571]
Biochimie	Inhibition of nuclease activity by a splice-switching oligonucleotide targeting deoxyribonuclease 1 mRNA prevents apoptosis progression and prolong viability of?… [Pubmed: 32315661]
Scientific Reports	Biomarkers of neutrophil extracellular traps (NETs) and nitric oxide-(NO)-dependent oxidative stress in women who miscarried [Pubmed: 32753622]
J Am Heart Assoc	DNase 1 Protects From Increased Thrombin Generation and Venous Thrombosis During Aging: Cross‐Sectional Study in Mice and Humans [Pubmed:35023342]

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編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
UAPB127Hu01	脫氧核糖核酸酶Ⅰ(DNASE1)活性蛋白	Cell?culture;?Activity?Assays.
URPB127Hu01	脫氧核糖核酸酶Ⅰ(DNASE1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAB127Hu01	脫氧核糖核酸酶Ⅰ(DNASE1)多克隆抗體	WB; IHC
ULAB127Hu81	脫氧核糖核酸酶Ⅰ(DNASE1)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
ULAB127Hu71	脫氧核糖核酸酶Ⅰ(DNASE1)多克隆抗體(生物素標(biāo)記)	WB
UMAB127Hu22	脫氧核糖核酸酶Ⅰ(DNASE1)單克隆抗體	WB,IHC
UMAB127Hu21	脫氧核糖核酸酶Ⅰ(DNASE1)單克隆抗體	WB; IHC; ICC; IP.
ULAB127Hu82	脫氧核糖核酸酶Ⅰ(DNASE1)單克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
ULAB127Hu72	脫氧核糖核酸酶Ⅰ(DNASE1)單克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
USEB127Hu	脫氧核糖核酸酶Ⅰ(DNASE1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB127Hu	脫氧核糖核酸酶Ⅰ(DNASE1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.