葡萄糖6磷酸脫氫酶(G6PD)多克隆抗體
Polyclonal Antibody to Glucose-6-phosphate Dehydrogenase (G6PD)
Glucose-6-phosphate 1-dehydrogenase
- 編號UPAA716Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA716Hu01-葡萄糖6磷酸脫氫酶(G6PD)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種-
- 應用WB; IHC; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 284 ¥ 664 ¥ 948 ¥ 2370 ¥ 9480
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human G6PD Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Lymph node Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human G6PD Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Lymph node Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human G6PD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Spleen Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human G6PD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: LO2 cell lysate; Primary Ab: 0.2μg/ml Rabbit Anti-Human G6PD Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human G6PD Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: G6PD knockout Hela cell lysate;
Predicted MW: 62kd
Observed MW: 60kd
Primary Ab: 1μg/ml Rabbit Anti-Human G6PD Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human G6PD Polyclonal Antibody (Catalog PAA716Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對G6PD的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別G6PD。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA716Hu02 | 葡萄糖6磷酸脫氫酶(G6PD)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA716Hu01 | 葡萄糖6磷酸脫氫酶(G6PD)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA716Hu01 | 葡萄糖6磷酸脫氫酶(G6PD)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA716Hu02 | 葡萄糖6磷酸脫氫酶(G6PD)多克隆抗體 | WB |
| UPAA716Hu01 | 葡萄糖6磷酸脫氫酶(G6PD)多克隆抗體 | WB; IHC; FCM |
| ULAA716Hu71 | 葡萄糖6磷酸脫氫酶(G6PD)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA716Hu22 | 葡萄糖6磷酸脫氫酶(G6PD)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA716Hu | 葡萄糖6磷酸脫氫酶(G6PD)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA716Hu | 葡萄糖6磷酸脫氫酶(G6PD)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| UKSA716Hu01 | 葡萄糖6磷酸脫氫酶(G6PD)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Artificial Organs | Experimental Research With Synthetic Copolymer-Coated Cardiopulmonary Bypass Circuits: Inflammatory and Thrombogenicity Analysis [Wiley: source] |
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