核仁磷酸蛋白(NPM1)多克隆抗體
Polyclonal Antibody to Nucleophosmin 1 (NPM1)
B23; NPM; Nucleolar Phosphoprotein B23; Numatrin; Nucleophosmin/Nucleoplasmin Family,Member 1; Nucleolar protein NO38
- 編號UPAC664Mu02
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPC664Mu02-核仁磷酸蛋白(NPM1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Homo sapiens (Human，人)
- 應用WB; IHC; ICC/IF
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 324 ¥ 756 ¥ 1080 ¥ 2700 ¥ 10800
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Samples: Mouse Adipose tissue Tissue; Primary Ab: 30μg/ml Rabbit Anti-Mouse NPM Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Stomach Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Small intestine Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Pancreas Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Uterus Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Cerebrum Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 30μg/ml Rabbit Anti-Mouse NPM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Uterus Tissue
Primary Ab: 30μg/ml Rabbit Anti-Mouse NPM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 7μg/ml Rabbit Anti-Mouse NPM1 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant NPM, Mouse.
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Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Jurkat cell lysate; Lane3: K562 cell lysate
Primary Ab: 1μg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: NPM knockout Jurkat cell lysate;
Predicted MW: 33kd
Observed MW: 38kd
Primary Ab: 1μg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對NPM1的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別NPM1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunofluorescence:5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
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相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPC664Mu01 | 核仁磷酸蛋白(NPM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC664Mu02 | 核仁磷酸蛋白(NPM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC664Mu02 | 核仁磷酸蛋白(NPM1)多克隆抗體 | WB; IHC; ICC/IF |
| UPAC664Mu01 | 核仁磷酸蛋白(NPM1)多克隆抗體 | WB; IHC |
| ULAC664Mu71 | 核仁磷酸蛋白(NPM1)多克隆抗體(生物素標記) | WB |
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| ULMC664Mu | 核仁磷酸蛋白(NPM1)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
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