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M2-型丙酮酸激酶(PKM2)多克隆抗體

Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle

  • M2-型丙酮酸激酶(PKM2)多克隆抗體 產品包裝(模擬)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 產品包裝(模擬)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
    Samples: Human Skin cancer Tissue;
    Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
    Sample: Mouse Lung Tissue
    Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
    Samples: Human Prostate cancer Tissue;
    Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
    Sample: Mouse Testis Tissue
    Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
    Sample: Mouse Oviduct Tissue
    Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 FITC staining on IF;<br/>Samples: Human MCF7 cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human PKM2 Antibody<br/>Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 AF488 staining on IF;<br/>Sample: Hela cell<br/>Primary Ab: 20μg/ml Rabbit Anti-Human PKM2 Antibody<br/>Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb11)
  • PAA588Hu01.jpg Figure. Western Blot; Sample: Recombinant PKM2, Human.
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 Western Blot; Sample: Human K562 cell lysate;
    Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: PKM2 knockout MCF7 cell lysate;
    Predicted MW: 56,58kd
    Observed MW: 60kd
    Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human PKM2 Polyclonal Antibody (Catalog PAA588Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • M2-型丙酮酸激酶(PKM2)多克隆抗體 Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human PKM2 Polyclonal Antibody (Catalog PAA588Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

該抗體是針對PKM2的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別PKM2。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;

Immunofluorescence:5-20μg/mL;
Flow cytometry: 20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。

贈品

相關產品

編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
URPA588Hu01 M2-型丙酮酸激酶(PKM2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPA588Hu02 M2-型丙酮酸激酶(PKM2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPA588Hu03 M2-型丙酮酸激酶(PKM2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPA588Hu02 M2-型丙酮酸激酶(PKM2)活性蛋白 Cell?culture;?Activity?Assays.
UPAA588Hu02 M2-型丙酮酸激酶(PKM2)多克隆抗體 WB; IHC; ICC/IF; FCM
UPAA588Hu03 M2-型丙酮酸激酶(PKM2)多克隆抗體 WB; IHC; ICC/IF
UPAA588Hu01 M2-型丙酮酸激酶(PKM2)多克隆抗體 WB; IHC; ICC/IF; FCM
UFAA588Hu01 抗M2-型丙酮酸激酶(PKM2)多克隆抗體 FCM
UFAA588Hu03 抗M2-型丙酮酸激酶(PKM2)多克隆抗體 FCM
UMAA588Hu24 M2-型丙酮酸激酶(PKM2)單克隆抗體 WB
UMAA588Hu22 M2-型丙酮酸激酶(PKM2)單克隆抗體 WB
UMAA588Hu23 M2-型丙酮酸激酶(PKM2)單克隆抗體 WB; ICC/IF
UMAA588Hu21 M2-型丙酮酸激酶(PKM2)單克隆抗體 WB
USEA588Hu M2-型丙酮酸激酶(PKM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA588Hu M2-型丙酮酸激酶(PKM2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Cancer Research Antigen Presentation by Dendritic Cells in Tumors Is Disrupted by Altered Metabolism that Involves Pyruvate Kinase M2 and Its Interaction with SOCS3 [AACR: 70189]
Molecular BioSystems Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer [Pubmed:25483567]
Tumour Biol M2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cells [PubMed: 26404132]
Oncotarget Identification of a serum biomarker panel for the differential diagnosis of cholangiocarcinoma and primary sclerosing cholangitis [Pubmed:29707118]
Journal of?Neurotrauma Acteoside improves muscle atrophy and motor function by inducing new myokine secretion in chronic spinal cord injury [Pubmed: 30318996]
Cell Reports Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway [Pubmed: 32049010]
Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC) []
Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins []
Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212]
Life (Basel) Putative Association between Low Baseline Gene Expression in the Peripheral Blood and Clinical Remission in Rheumatoid Arthritis Patients Treated with Tofacitinib [34947916]
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