粘蛋白5AC(MUC5AC)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Mucin 5 Subtype AC (MUC5AC)
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
- 編號USCA756Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長2h, 40min
- 檢測范圍13.7-10,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.3pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1814 ¥ 2592 ¥ 11664 ¥ 22032 ¥ 181440
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測粘蛋白5AC(MUC5AC)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的粘蛋白5AC(MUC5AC)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| EDTA plasma(n=5) | 79-104 | 92 |
| heparin plasma(n=5) | 92-101 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的粘蛋白5AC(MUC5AC)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中粘蛋白5AC(MUC5AC)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| EDTA plasma(n=5) | 78-102% | 86-101% | 92-105% | 79-94% |
| heparin plasma(n=5) | 81-95% | 93-101% | 86-94% | 82-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實(shí)驗(yàn)原理
將粘蛋白5AC(MUC5AC)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的粘蛋白5AC(MUC5AC)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的粘蛋白5AC(MUC5AC)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強(qiáng)度和樣品中的粘蛋白5AC(MUC5AC)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計(jì)算樣品濃度。
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| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA756Mu02 | 粘蛋白5AC(MUC5AC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA756Mu01 | 粘蛋白5AC(MUC5AC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA756Mu03 | 粘蛋白5AC(MUC5AC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA756Mu01 | 粘蛋白5AC(MUC5AC)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA756Mu02 | 粘蛋白5AC(MUC5AC)多克隆抗體 | IHC; ICC/IF |
| UPAA756Mu03 | 粘蛋白5AC(MUC5AC)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA756Mu81 | 粘蛋白5AC(MUC5AC)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA756Mu71 | 粘蛋白5AC(MUC5AC)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UIEA756Mu | 粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA756Mu | 粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA756Mu | 粘蛋白5AC(MUC5AC)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA756Mu | 粘蛋白5AC(MUC5AC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA756Mu01 | 粘蛋白5AC(MUC5AC)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| European Journal of Pharmacology | Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways [ScienceDirect: S0014299912005523] |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905] |
| Respiratory Researh | Pyocyanin-induced mucin production is associated with redox modification of FOXA2 [PubMed: PMC3765780] |
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| BASIC INVESTIGATION | Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases [pubmed:28644234] |
| International Immunopharmacology | Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathway [pubmed:28550735] |
| Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой [10.20538/1682-0363-2017-2-146-158] | |
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| Inflammation | Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in?… [Pubmed:29349683] |
| Pulmonary Pharmacology and Therapeutics | In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extracts [Pubmed:29698781] |
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