誘導型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)
NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2
- 編號USEA837Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.055ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1323 ¥ 1890 ¥ 8505 ¥ 16065 ¥ 132300
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測誘導型一氧化氮合酶(NOS2)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的誘導型一氧化氮合酶(NOS2)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 92-101 | 95 |
| EDTA plasma(n=5) | 90-99 | 93 |
| heparin plasma(n=5) | 93-101 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的誘導型一氧化氮合酶(NOS2)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中誘導型一氧化氮合酶(NOS2)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-104% | 80-96% | 97-105% | 94-105% |
| EDTA plasma(n=5) | 93-101% | 99-105% | 87-97% | 88-96% |
| heparin plasma(n=5) | 89-104% | 85-101% | 90-97% | 79-88% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將誘導型一氧化氮合酶(NOS2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的誘導型一氧化氮合酶(NOS2)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的誘導型一氧化氮合酶(NOS2)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的誘導型一氧化氮合酶(NOS2)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA837Hu03 | 誘導型一氧化氮合酶(NOS2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA837Hu02 | 誘導型一氧化氮合酶(NOS2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA837Hu01 | 誘導型一氧化氮合酶(NOS2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA837Hu03 | 誘導型一氧化氮合酶(NOS2)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA837Hu01 | 誘導型一氧化氮合酶(NOS2)多克隆抗體 | WB; IHC |
| UPAA837Hu02 | 誘導型一氧化氮合酶(NOS2)多克隆抗體 | WB; IHC |
| UFAA837Hu01 | 抗誘導型一氧化氮合酶(NOS2)多克隆抗體 | FCM |
| ULAA837Hu71 | 誘導型一氧化氮合酶(NOS2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA837Hu23 | 誘導型一氧化氮合酶(NOS2)單克隆抗體 | IHC |
| UMAA837Hu21 | 誘導型一氧化氮合酶(NOS2)單克隆抗體 | WB |
| UMAA837Hu25 | 誘導型一氧化氮合酶(NOS2)單克隆抗體 | WB |
| UMAA837Hu22 | 誘導型一氧化氮合酶(NOS2)單克隆抗體 | WB; IHC |
| UMAA837Hu27 | 誘導型一氧化氮合酶(NOS2)單克隆抗體 | ICC/IF |
| UMAA837Hu29 | 誘導型一氧化氮合酶(NOS2)單克隆抗體 | ICC/IF |
| UMAA837Hu24 | 誘導型一氧化氮合酶(NOS2)單克隆抗體 | IHC |
| UFAA837Hu02 | 抗誘導型一氧化氮合酶(NOS2)單克隆抗體 | FCM |
| UFAA837Hu04 | 抗誘導型一氧化氮合酶(NOS2)單克隆抗體 | FCM |
| UHEA837Hu | 誘導型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA837Hu | 誘導型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UWEA837Hu | 誘導型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA837Hu | 誘導型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA837Hu01 | 誘導型一氧化氮合酶(NOS2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice [PubMed: 20713153] |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression [ScienceDirect: S0925443911001323] |
| FEBS Journal | Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta [Wiley: source] |
| Gene | Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors [ScienceDirect: S0378111911007347] |
| PLoS One | A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide [PubMed: 23359814] |
| PLoS ONE | Regulation of Microglia Activity by Glaucocalyxin-A: Attenuation of Lipopolysaccharide-Stimulated Neuroinflammation through NF-κB and p38 MAPK Signaling Pathways [PlosOne: Source] |
| Journal of Cellular Biochemistry | The inhibition of hyaluronan degradation reduced pro-inflammatory cytokines in mouse synovial fibroblasts subjected to collagen-induced arthritis. [PubMed: 22234777] |
| International Immunopharmacology | Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis [Pubmed: 24126114] |
| Integrative Cancer Therapies | Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits [Pubmed: 24013642] |
| Food and Chemical Toxicology | Inhibition of UVB-induced skin phototoxicity by a grape seed extract as modulator of nitrosative stress, ERK/NF-kB signaling pathway and apoptosis, in SKH-1 mice [Pubmed: 23567245] |
| Experimental Gerontology | Age-related changes in ADMA–DDAH–NO pathway in rat liver subjected to partial ischemia followed by global reperfusion [ScienceDirect: S0531556513003331] |
| International Immunopharmacology | Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits [ScienceDirect: S1567576913004153] |
| INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH | L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION [Tdmu:Source] |
| Molecular Vision | Effects of topically applied tocotrienol on cataractogenesis and lens redox status in galactosemic rats [Pubmed:24940038] |
| Journal of Environmental Pathology, Toxicology and Oncology | Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways [Pubmed:24941292] |
| Eur J Cancer Prev. | Utility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies [Pubmed:25222193] |
| Cell and Tissue Research | Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes [Pubmed:25673209] |
| Ann N Y Acad Sci.? | Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis [Pubmed:5243883] |
| Molecular Medicine Reports | Resveratrol ameliorates oxidative stress and inhibits aquaporin 4 expression following rat cerebral ischemia-reperfusion injury [PubMed: 26458999] |
| Chem Biol Interact | Apocynin protects against ethanol-induced gastric ulcer in rats by attenuating the upregulation of NADPH oxidases 1 and 4 [PubMed: 26522475] |
| Journal of Environmental Pathology, Toxicology and Oncology | Protective Effect of [PubMed: 26349607] |
| Journal of Immunotoxicology | Acacia ferruginea inhibits inflammation by regulating inflammatory iNOS and COX-2 [PubMed: 25738525] |
| Cancer Medicine | Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease [Pubmed:27109254] |
| Journal of Natural Medicines | Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice [Pubmed:26972100] |
| Cell Mol Immunol? | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496] |
| Медицина и здравоохранение | Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу [article:n] |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome [pubmed:27538909] |
| Taylor & Francis | Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation [09540105.2017.1306494] |
| Molecular Vision | Mechanism of the anticataract effect of liposomal MgT in galactose-fed rats. [pubmed:27440992] |
| Journal of Natural Medicines | Anti-oxidative,?anti-secretory?and?anti-inflammatory?activities?of the?extract?from the?root?bark?ofLycium?chinense?(Cortex Lycii) against gastric ulcer in mice. [pubmed:26972100] |
| Applied?Biochemistry?and?Biotechnology | Protective?Effect?of?Zingiber?officinale?Against?Dalton's?Lymphoma?Ascites?Tumour?byRegulating?Inflammatory?Mediator?and?Cytokines. [pubmed:27435276] |
| Cell Mol Neurobiol | Posttraumatic Stress Disorder Disturbs Coronary Tone and Its Regulatory Mechanisms [pubmed:28676988] |
| Microbial Immunology | Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota [pubmed:28713364] |
| Biomedicine & Pharmacotherapy | Protective effect of Averrhoa bilimbi L. fruit extract on ulcerative colitis in wistar rats via regulation of inflammatory mediators and cytokines [pubmed:28531922] |
| The Royal Society of Chemistry | Anti-ulcerogenic effect of KFP-H008 against ethanol-induced gastric ulcer via p38 MAPK/NF-κB pathway [10.1039/C7RA08879E] |
| Acta Pharmacologica Sinica | Cis-stilbene glucoside in Polygonum multiflorum induces immunological idiosyncratic hepatotoxicity in LPS-treated rats by suppressing PPAR-γ. [pubmed:28649126] |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects [pubmed:29102164] |
| Molecules | Melatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy [Pubmed:29547569] |
| European?Journal of Pharmacology? | Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat [Pubmed:29518393] |
| Regulatory Toxicology and Pharmacology | Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and?… [Pubmed:29678767] |
| Pharmacological Reports | The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder [10.1016:j.pharep.2018.06.001] |
| Clinical?And Experimental Pharmacology And Physiology? | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336] |
| St??enia ?ródb?onkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ci??arnych pal?cych tytoń–badania wst?pne [] | |
| Journal of?Allergy?and?Clinical?Immunology | Exosome swarms eliminate airway pathogens and provide passive epithelial immunoprotection through nitric oxide [Pubmed: 30442371] |
| Metabolic?Brain?Disease | Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms [Pubmed: 30421246] |
| PLoS?One | Intraphagolysosomal conditions predispose to Staphylococcus epidermidis small colony variants persistence in macrophages [Pubmed: 30412620] |
| The?Eurasian?Journal of Medicine | Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats [Doi: 10.5152/eurasianjmed.2018.18160] |
| PLoS One | Macrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injury [Pubmed: 30571701] |
| International Journal of Environmental Research and?Public?Health | Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women [Pubmed: 30513890] |
| Journal of Functional Foods | Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments [] |
| Particle and?Fibre?Toxicology | Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathway [Pubmed: 30975181] |
| ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ?… [] | |
| Journal of Cosmetic Dermatology | Whitening effects of cosmetic formulation in the vascular component of skin pigmentation [Pubmed: 31074159] |
| regional hemodynamics and microcirculation | Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility [] |
| Life Sci | Cilostazol mitigates mesenteric ischemia/reperfusion-induced lung lesion: Contribution of PPAR-|?, NF-|êB, and STAT3 crosstalk [33310046] |
| PLoS One | Correlation analysis of the proportion of monocytic myeloid-derived suppressor cells in colorectal cancer patients [33370317] |
| Biotechnol Rep | Targeting NF-|êB mediated cell signaling pathway and inflammatory mediators by 1, 2-diazole in A549 cells in vitro [33598414] |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease [33995926] |
| Fermented Foods and Microbes Related to Health | Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A|?40 and Streptozotocin??- [] |
| Ya?lanma ve Hipertansiyonda Yeni bir Endoltelyal Disfonksiyon Belirteci: ??z¨1nebilir Lektin Benzeri Okside D¨1?¨1k Dansiteli Lipoprotein-1 [] | |
| Fermentation | Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A|?40 and Streptozotocin-Induced Memory Deficit via Suppressing Alzheimer?ˉs Disease Risk Factors and Increasing Magnesium Uptake of Brain [] |
| Drug Chem Toxicol | Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline?… [34167392] |
| Frontiers in Cardiovascular Medicine | The Effects of Intermittent Hypoxic–Hyperoxic Exposures on Lipid Profile and Inflammation in Patients With Metabolic Syndrome [34513946] |
| Nutrients | Erinacine A-Enriched Hericium erinaceus Mycelium Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice [34684662] |
| biomolecules | Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men [34356664] |
| Hum Exp Toxicol | Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat [34155936] |
| J Ethnopharmacol | Post-stroke treatment of storax improves long-term outcomes of stroke in rats [34343648] |
| Phytomedicine | A multidimensional strategy for uncovering comprehensive quality markers of Schisandra chinensis (Turcz.) Baill based on pharmacodynamics and chemical?… [] |
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