分泌型卷曲相關(guān)蛋白5(SFRP5)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)
SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b
- 編號USEC842Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍125-8000pg/mL
- 靈敏度最小可檢測劑量小于等于47pg/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1596 ¥ 2280 ¥ 10260 ¥ 19380 ¥ 159600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測分泌型卷曲相關(guān)蛋白5(SFRP5)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的分泌型卷曲相關(guān)蛋白5(SFRP5)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-101 | 98 |
| EDTA plasma(n=5) | 78-96 | 84 |
| heparin plasma(n=5) | 79-98 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的分泌型卷曲相關(guān)蛋白5(SFRP5)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中分泌型卷曲相關(guān)蛋白5(SFRP5)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-105% | 83-90% | 97-105% | 87-96% |
| EDTA plasma(n=5) | 81-93% | 87-98% | 89-103% | 91-103% |
| heparin plasma(n=5) | 98-105% | 78-90% | 80-102% | 88-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將分泌型卷曲相關(guān)蛋白5(SFRP5)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的分泌型卷曲相關(guān)蛋白5(SFRP5)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的分泌型卷曲相關(guān)蛋白5(SFRP5)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的分泌型卷曲相關(guān)蛋白5(SFRP5)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC842Ra01 | 分泌型卷曲相關(guān)蛋白5(SFRP5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC842Ra01 | 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體 | WB |
| ULAC842Ra71 | 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAC842Ra21 | 分泌型卷曲相關(guān)蛋白5(SFRP5)單克隆抗體 | WB; IHC; ICC; IP. |
| USEC842Ra | 分泌型卷曲相關(guān)蛋白5(SFRP5)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC842Ra | 分泌型卷曲相關(guān)蛋白5(SFRP5)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Diabetes Research and Clinical Practice | Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance [PubMed: 23290274] |
| European Journal of Clinical Investigation | Sfrp5 correlates with insulin resistance and oxidative stress [PubMed: 23398169] |
| Pediatric Research | Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children [Pubmed: 24603290] |
| Clin Endocrinol (Oxf). | SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children [Pubmed: 24330025] |
| The Journal of Clinical Endocrinology & Metabolism | Circulating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humans [Pubmed: 23185036] |
| Atherosclerosis | Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease [Pubmed: 24530778] |
| PLoS One. | Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation [Pubmed:Pmc4072672] |
| J Clin Endocrinol Metab. | Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation [Pubmed:24840810] |
| Oncology Letters | Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma [Pubmed:25120720] |
| Diabetes Metab Res Rev. | Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls [Pubmed:25139699] |
| Clinical & Experimental Immunology | The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis [Pubmed:25382802] |
| Diabetes Metab Res Rev | Circulating SFRP5 levels are elevated in drug-na [PubMed: 25139699] |
| J Matern Fetal Neonatal Med | Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels [PubMed: 26100762] |
| Eur J Clin Invest | Sfrp5 associates with beta-cell function in humans [Pubmed:27019073] |
| Osteoporosis International | Bone marrow sFRP5 level is negatively associated with bone formation markers [pubmed:27986984] |
| Diabetes/Metabolism Research and Reviews | Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults [pubmed:28303637] |
| Cardiovascular Diabetology | Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study [pubmed:28851362] |
| Arteriosclerosis, Thrombosis, and?Vascular Biology? | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With?… [Pubmed:29674475] |
| Obesity | Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans [Pubmed:29427376] |
| J Bas Res Med Sci | Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats [article-1-352-en.pdf] |
| Molecular?Biology?Reports | The effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndrome [Pubmed: 30194556] |
| Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling [] | |
| British?Journal Of Nutrition | A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile [Pubmed: 30375290] |
| J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans [Pubmed: 30137542] |
| Frontiers in Endocrinology | Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients [Pubmed: 32300333] |
| CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease [Pubmed: 33188773] |
| Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … [Pubmed: 32491086] |
| Kidney Blood Press Res | Secreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway [34469882] |
| Cytokine | Association of serum uromodulin with adipokines in dependence of type 2 diabetes [34920231] |
| PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study [Pubmed:34990470] |
| JMIR Res Protoc | Investigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition?… [Pubmed:35475790] |
| Diabetology | Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop?… [] |
