低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)
High Sensitive ELISA Kit for Low Density Lipoprotein (LDL)
- 編號UHEB107Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.78-50ng/mL
- 靈敏度最小可檢測劑量小于等于0.29ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 924 ¥ 1320 ¥ 5940 ¥ 11220 ¥ 92400
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 92-101 | 96 |
| EDTA plasma(n=5) | 80-93 | 88 |
| heparin plasma(n=5) | 82-104 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-94% | 85-105% | 94-103% | 91-99% |
| EDTA plasma(n=5) | 89-99% | 92-105% | 82-98% | 80-89% |
| heparin plasma(n=5) | 90-104% | 82-101% | 95-105% | 82-94% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UNPB107Hu01 | 低密度脂蛋白(LDL)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB107Hu01 | 低密度脂蛋白(LDL)多克隆抗體 | IHC |
| ULAB107Hu71 | 低密度脂蛋白(LDL)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UHEB107Hu | 低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEB107Hu | 低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB107Hu | 低密度脂蛋白(LDL)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB107Hu | 低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB107Hu01 | 低密度脂蛋白(LDL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PloS one | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. [NCBI: PMC3896367] |
| PLoS One. | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL [Pubmed:24465540] |
| J Lipid Res | PCSK9 deficiency unmasks a sex-and tissue-specific subcellular distribution of the LDL and VLDL receptors in mice [PubMed: 26323289] |
| Neuroscience | 27-Hydroxycholesterol contributes to disruptive effects on learning and memory by modulating cholesterol metabolism in the rat brain [PubMed: 25987203] |
| The Journal of Nutritional Biochemistry | Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice [Pubmed:27142749] |
| ?Journal of Functional Foods | Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese mice [S1756464617300555] |
| Int J Mol Sci. | Combined Effects of Curcumin and Lycopene or Bixin in Yoghurt on Inhibition of LDL Oxidation and Increases in HDL and Paraoxonase Levels in Streptozotocin-Diabetic Rats. [pubmed:28333071] |
| Journal of Surgical Arts/Cerrahi Sanatlar Dergisi | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. Hiperlipidemili ve … [163] |
| Journal of Functional Foods | Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese mice [10.1016:j.jff.2017.01.044] |
| Microvascular Research | Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients [pubmed:28954218] |
| Biochemical and Biophysical Research Communications | Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release [pubmed:29146189] |
| Journal of Nutritional Science and Vitaminology (Tokyo) | Anthocyanin Cyanidin-3-Glucoside Attenuates Platelet Granule Release in Mice Fed High-Fat Diets. [pubmed:28978870] |
| Environmental?Science?and Pollution?Research | Bifenthrin exerts proatherogenic effects via arterial accumulation of native and oxidized LDL in rats: the beneficial role of vitamin E and selenium [Pubmed: 30465240] |
| American Journal of Translational?Research | Alteration of circulating innate lymphoid cells in patients with atherosclerotic cerebral infarction [Pubmed: 30662674] |
| Nature Communications | ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy [Pubmed: 31358770] |
| Diabetes Metab Syndr Obes | ?°Adjusting Internal Organs and Dredging Channel?± Electroacupuncture Ameliorates Insulin Resistance in Type 2 Diabetes Mellitus by Regulating the Intestinal??- [34135611] |
| Evid Based Complement Alternat Med | Effect of Acupoint Embedding on Serum Leptin and Hypothalamus Leptin Receptor Expression in Rats with Simple Obesity [34659432] |
| Ecotoxicol Environ Saf | High-fat diet-induced aggravation of cardiovascular impairment in permethrin-treated Wistar rats [34224971] |
| Int J Food Sci Nutr | Brown and golden flaxseed reduce intestinal permeability and endotoxemia, and improve the lipid profile in perimenopausal overweight women [Pubmed:35311432] |
