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己糖激酶2(HK2)多克隆抗體

FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 10μg/ml Rabbit Anti-Human HK2 Antibody Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 10μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

WB圖
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: HK2 knockout MCF7 cell lysate;
Predicted MW: 102kd
Observed MW: 102kd
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human A431 cell lysate;
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human HK2 Polyclonal Antibody (Catalog PAD352Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

該抗體是針對HK2的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別HK2。

Western blotting: 0.01-5μg/mL;
Immunocytochemistry: 5-20μg/mL;

Flow cytometry:20μg/mL;

Optimal working dilutions must be determined by end user.

經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。

雜志	參考文獻
Anti-Cancer Drugs	3-Bromopyruvate enhances TRAIL-induced apoptosis in human nasopharyngeal carcinoma cells through CHOP-dependent upregulation of TRAIL-R2. [10.1097/CAD.0000000000000502]
	Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC) []
J Cell Mol Med	Huaier shows anti?\cancer activities by inhibition of cell growth, migration and energy metabolism in lung cancer through PI3K/AKT/HIF?\1\|á pathway [33377619]

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編號	適用物種：Homo sapiens (Human，人)	應用(僅供研究使用，不用于臨床診斷！)
URPD352Hu01	己糖激酶2(HK2)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPD352Hu02	己糖激酶2(HK2)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPD352Hu01	己糖激酶2(HK2)活性蛋白	Cell?culture;?Activity?Assays.
UPAD352Hu01	己糖激酶2(HK2)多克隆抗體	WB; IHC
UPAD352Hu02	己糖激酶2(HK2)多克隆抗體	WB; ICC/IF; FCM
UMAD352Hu21	己糖激酶2(HK2)單克隆抗體	WB; IHC; ICC; IP.
USED352Hu	己糖激酶2(HK2)檢測試劑盒(酶聯免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMD352Hu	己糖激酶2(HK2)等多因子檢測試劑盒(流式熒光發光法)	FLIA Kit for Antigen Detection.